DNA Walking Service Launched DNA Walking Service A kit to identify unknown sequences adjacent to upstream or downstream known sequencesDNA Walking SpeedUpTM kit provides information about unknown sequences adjacent to upstream or downstream known sequences. Previous methods commonly used for this purpose include inverse PCR (IPCR), ligation-mediated PCR (LM-PCR) and randomly primed PCR (RP-PCR). Although these methods have been used efficiently and have proven to be effective, they are still hampered by laborious steps such as restriction enzyme digestion, adaptor ligation, and purification procedures. Moreover, arbitrary pimers in RP-PCR often result in non-specific products due to non-specific annealing. These drawbacks of existing genomic walking methods can be overcome by employing Seegene's proprietary ACPTM (Annealing Control Primer) Seegene developed DNA walking technology by incorporating ACPTM (Annealing Control Primer) and DPOTM (Dual Priming Oligonucleotide) technologies, which maximize the specificities of short primers and target specific primers, respectively, and furnishes optimized data about unknown sequences. Note! DNA WalkingTM kit II, which provides widen application and higher success ratio than DNA WalkingTM kit I, has been recently released.
GeneFishing™ Kit LaunchA kit to discover differentially expressed genes in two or more samples. * Introduction DEG stands for Differentially Expressed Gene and refers to all genes that are expressed differentially at the mRNA level in two or more samples. GeneFishingTM Technology is a new and unique approach that is capable of identifying only authentic Differentially Expressed Genes (DEGs) in two or more nucleic acid samples. This technology dramatically improves the specificity of PCR amplification, enabling researchers to find authentic PCR products. GeneFishingTM is the first application of Seegene's proprietary technology which is based on innovative primer design (Annealing Control Primer, ACPTM, patent pending). * Advantages & Features 1. Maximum specificity - No false positives!! 2. Guaranteed reproducibility 3. Simplicity - No specific skills required 4. Extensive range of PCR products 5. A fast and economical method 6. No PAGE & radioactivity/fluorescent tags (agarose detection)