Anyplex™ MERS-CoV (upE & orf1a) Real-time Detection is a multiplex one-step real-time RT-PCR assay targeting upstream of the E protein gene (upE) and orf1a gene for screening and confirmation of MERS-CoV. The assay for the upE target is considered highly sensitive, with the orf1a assay considered of equal sensitivity within 2.2 hours after extraction.
Key Features and Benefits
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Multiplex real-time PCR
Detection of upE and orf1a gene for screening and confirmation of MERS-CoV
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Powerful performance with unique technology
Multiplex real-time PCR with high sensitivity and specificity by utilization of DPO™ technology
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Informative assay
Assistant of appropriate treatment and management for co-infection
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Whole process validation
Whole process validation from extraction to PCR by whole process control
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User-friendly workflow
Convenient workflow using Seegene’s automated one platform
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UDG system
Utilization of the UDG system to prevent carry-over contamination
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Automatic data analyzer
Automated data interpretation and LIS interlocking with Seegene Viewer
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Analytes
Anyplex™
MERS-CoV(upE & orf1a) Real-time Detection
- orf1a gene (orf1a)
- upE gene (upE)
- Internal Control (IC)
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Specimens
- Sputum
- Nasopharyngeal swab
- Nasopharyngeal aspirate
- Bronchoalveolar lavage
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Ordering Information
Result
The result represents detection that orf1a gene is 32.19 Ct value in the FAM channel, upE gene is 31.50 Ct value in the HEX channel.
Note
[1] For use with Seegene NIMBUS & STARlet only