Anyplex™ MERS-CoV (upE & orf1a) Real-time Detection is a multiplex one-step real-time RT-PCR assay targeting upstream of the E protein gene (upE) and orf1a gene for screening and confirmation of MERS-CoV. The assay for the upE target is considered highly sensitive, with the orf1a assay considered of equal sensitivity within 2.2 hours after extraction.
Detection of upE and orf1a gene for screening and confirmation of MERS-CoV
Convenient workflow using Seegene’s automated one platform
Utilization of the UDG system to prevent carry-over contamination
Automated data interpretation and LIS interlocking with Seegene Viewer
Multiplex PCR with high sensitivity and specificity by applying DPO™ technology
Assistant of appropriate treatment and management for co-infection
Whole process validation from extraction to PCR by whole process control
The result represents detection that orf1a gene is 32.19 Ct value in the FAM channel, upE gene is 31.50 Ct value in the HEX channel.
 For use with Seegene NIMBUS & STARlet only