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  • Real-time
  • CE-IVD

Anyplex™ II
RB5 Detection

Simultaneous detection of 5 respiratory bacteria by real-time PCR

Anyplex™Ⅱ RB5 Detection simultaneously detects and differentiates 5 respiratory bacteria in a single reaction. Based on Seegene’s proprietary DPO™ and TOCE™ technologies, this multiplex assay performs on  real-time PCR instruments and rapidly and simultaneously provide information on the infected CAP-related pathogens as well as two causative agents of whooping cough - Bordetella pertussis (BP) and Bordetella parapertussis (BPP) with high sensitivity and specificity.

Key Features and Benefits

  • Proper patient care

    Quick and proper treatment provided by accurate test results

  • Multiplex real-time PCR

    Detection of 5 respiratory bacteria in a single reaction

  • User-friendly workflow

    Convenient workflow using Seegene’s automated one platform

  • Quantitative analysis

    Quantitative analysis by cyclic-CMTA

  • UDG system

    Utilization of the UDG system to prevent carry-over contamination

  • Powerful performance with unique technology

    Multiplex real-time PCR with high sensitivity and specificity by utilization of DPO™ and TOCE™ technologies

  • Automatic data analyzer

    Automated data interpretation and LIS interlocking with Seegene Viewer

  • Informative assay

    Assistant of appropriate treatment and management for co-infection

  • Whole process validation

    Whole process validation from extraction to PCR by whole process control

  • Analytes

    Anyplex™ II
    RB5 Detection

    • Bordetella parapertussis (BPP)
    • Bordetella pertussis (BP)
    • Chlamydophila pneumoniae (CP)
    • Legionella pneumophila (LP)
    • Mycoplasma pneumoniae (MP)
    • Internal Control (IC)
  • Specimens

    • Nasopharyngeal swab
    • Nasopharyngeal aspirate
    • Bronchoalveolar lavage
    • Sputum [1]
  • Ordering Information

    Cat No. / Size
    Anyplex™ II RB5 Detection
    RB7500Y / 50 rxns
    RB7500X / 100 rxns [2]


The result represents co-infection that LP is low(+) in the FAM channel, BP is intermediate (++) in the HEX channel.


[1] not applicable on NIMBUS IVD & STARTlet IVD

[2] For use with Seegene NIMBUS & STARlet only