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  • Real-time
  • CE-IVD

GI-Bacteria(II) Assay

Comprehensive assay for the detection and identification of 6 bacteria causing gastrointestinal tract infections using real-time PCR

Allplex™ Bacteria(II) Assay is a multiplex real-time PCR assay that detects Hypervirulent Clostridium difficile and E. Coil with typing in gastrointestinal tract infection. Based on Seegene’s proprietary MuDT™ technology, this assay reports multiple Ct values of each pathogen in a single channel using real-time PCR instrument.

Key Features and Benefits

  • Multiplex real-time PCR

    Detection and differentiation of 6 bacteria causing gastrointestinal pathogens in a single reaction

  • Proper patient care

    Accurate test results allow to quick and proper treatment

  • Multi-Ct in a single channel

    Individual Ct value of multiple analytes in a single channel of real-time PCR instrument (MuDT™ Technology)

  • Automatic data analyzer

    Automated data interpretation and LIS interlocking with Seegene Viewer

  • Proper patient care

    Quick and proper treatment provided by accurate test results

  • Informative assay

    Assistant of appropriate treatment and management for co-infection

  • UDG system

    Utilization of the UDG system to prevent carry-over contamination

  • Whole process validation

    Whole process validation from extraction to PCR by whole process control

  • User-friendly workflow

    Convenient workflow using Seegene’s automated one platform

  • Analytes

    GI-Bacteria(II) Assay

    • EAEC[1] ( aggR )
    • EPEC[2] (eaeA)
    • Escherichia coli O157 (E. coli O157)
    • ETEC[3] (lt/st)
    • Hypervirulent Clostridium difficile (CD hyper)
    • STEC[4] (stx1/2)
    • Internal Control (IC)
  • Specimen

    • Human stool
  • Ordering Information

    Cat No. / Size
    Allplex™ GI-Bacteria(II) Assay
    GI10183Z / 25 rxns [5]
    GI9702X / 100 rxns [5]


Multiple Ct value in a single channel

The result represents detection of stx1/stx2 and eaeA gene in FAM channel with Ct values of 29.91(Graph 1) and 39.43 (Graph 2), respectively.


[1] Enteroaggregative E. coli

[2] Enteropathogenic E. coli

[3] Enterotoxigenic E. coli

[4] Shiga toxin-producing E. coli

[5] For use with Seegene NIMBUS & STARlet only