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  • Real-time
    PCR
  • CE-IVD
    marked

Allplex™
Gastrointestinal Panel Assays

Comprehensive assay for the detection and identification of 25 gastrointestinal pathogens using one-step real-time RT-PCR

Allplex™ Gastrointestinal Panel Assays is a multiplex one-step real-time RT-PCR assay that detects and identifies 25 gastrointestinal pathogens including 6 viruses, 13 bacteria and 6 parasites simultaneously. Based on Seegene’s proprietary MuDT™ technology, this assay reports multiple Ct values of each pathogen in a single channel using real-time PCR instrument. Allplex™ Gastrointestinal Panel Assays allows faster, more reliable and comprehensive test results than any other products by combination with Seegene’s automation platforms. 

Key Features and Benefits

  • Multiplex real-time PCR

    Broad pathogens coverage of 6 viruses, 13 bacteria and 6 parasites causing gastrointestinal pathogens in a single reaction

  • Full coverage of causative pathogens

    Comprehensive assay for the detection and identification of 25 (gastrointestinal) pathogens

  • User-friendly workflow

    Convenient workflow using Seegene’s automated one platform

  • Efficient syndromic test

    The syndromic screening test for efficient and cost-effectiveness patient care

  • Proper patient care

    Accurate test results allow to quick and proper treatment

  • Multi-Ct in a single channel

    Individual Ct value of multiple analytes in a single channel of real-time PCR instrument (MuDT™ Technology)

  • Automatic data analyzer

    Automated data interpretation and LIS interlocking with Seegene Viewer

  • Powerful performance with unique technology

    Multiplex real-time PCR with high sensitivity and specificity by utilization of DPO™ and TOCE™ technologies

  • Informative assay

    Assistant of appropriate treatment and management for co-infection

  • UDG system

    Utilization of the UDG system to prevent carry-over contamination

  • Whole process validation

    Whole process validation from extraction to PCR by whole process control

  • Analytes

    Allplex™
    GI-Bacteria(II) Assay
    • EAEC[1] ( aggR )
    • EPEC[2] (eaeA)
    • Escherichia coli O157 (E. coli O157)
    • ETEC[3] (lt/st)
    • Hypervirulent Clostridium difficile (CD hyper)
    • STEC[4] (stx1/2)
    • Internal Control (IC)
  • Allplex™
    GI-Bacteria(I) Assay
    • Aeromonas spp. (Aer)
    • Campylobacter spp. (Cam)
    • Clostridium difficile toxin B (CdB)
    • Salmonella spp. (Sal)
    • Shigella spp./EIEC[5] (Sh/EI)
    • Vibrio spp. (Vib)
    • Yersinia enterocolitica (Yer)
    • Internal Control (IC)
  • Allplex™
    GI-Parasite Assay
    • Blastocystis hominis (BH)
    • Cryptosporidium spp. (CR)
    • Cyclospora cayetanensis (CC)
    • Dientamoeba fragilis (DF)
    • Entamoeba histolytica (EH)
    • Giardia lamblia (GL)
    • Internal Control (IC)
  • Allplex™
    GI-Virus Assay
    • Adenovirus (AdV)
    • Astrovirus (AstV)
    • Norovirus GI (NoV-GI)
    • Norovirus GII (NoV-GII)
    • Rotavirus (RotV)
    • Sapovirus (SV)
    • Internal Control (IC)
  • Specimen

    • Stool
  • Ordering Information

    Product
    Cat No. / Size
    Allplex™ GI-Bacteria(II) Assay
    GI10183Z / 25 rxns [6]
    GI9702Y / 50 rxns
    GI9702X / 100 rxns [6]
    Allplex™ GI-Bacteria(I) Assay
    GI10201Z / 25 rxns [6]
    GI9801Y / 50 rxns
    GI9801X / 100 rxns [6]
    Allplex™ GI-Parasite Assay
    GI10202Z / 25 rxns [6]
    GI9703Y / 50 rxns
    GI9703X / 100 rxns [6]
    Allplex™ GI-Virus Assay
    GI10184Z / 25 rxns [6]
    GI9701X / 100 rxns [6]

Result

Multiple Ct value in a single channel

The result represents the co-infection of B. hominis(BH) G. lamblia(GL) in FAM channel with Ct values of 20.10 (Graph 1) and 31.10 (Graph 2), respectively.

Publication

- Evaluation of the BioFire Gastrointestinal Panel to Detect Diarrheal Pathogens in Pediatric Patients

- Diagnosis of Acute Gastrointestinal Infections in Hospitalized Patients: A Molecular-Based Screening Approach

- Evaluation of the multiplex PCR Allplex-GI assay in the detection of bacterial pathogens in diarrheic stool samples

- A comparison of the Allplex™ bacterial and viral assays to conventional methods for detection of gastroenteritis agents

- Workflow optimization for syndromic diarrhea diagnosis using the molecular Seegene Allplex™ GI-Bacteria(I) assay

- Efficacy of stool multiplex ploymerase chain assay in adult patients with acute infectious diarrhea 

- Comparative Evaluation of Seegene Allplex Gastrointestinal, Luminex xTAG Gastrointestinal Pathogen Panel, and BD MAX Enteric Assays for Detection of Gastrointestinal Pathogens in Clinical Stool Specimens

- Comparative Performance of Eight PCR Methods to Detect Cryptosporidium Species

- One Health Approach to Zoonotic Parasites: Molecular Detection of Intestinal Protozoans in an Urban Population of Norway Rats, Rattus norvegicus, in Barcelona, Spain 

- Evaluation of the Allplex™ Gastrointestinal Panel-Parasite Assay for Protozoa Detection in Stool Samples : A Retrospective and Prospective Study

- Comparative performance evaluation of four commercial multiplex real-time PCR assays for the detection of the diarrhoea-causing protozoa Cryptosporidium spp. (hominis/parvum), Giardia duodenalis and Entamoeba histolytica

- Human Astrovirus Outbreak in a Daycare Center and Propagation among Household Contacts

- Virus Etiology, Diversity and Clinical Characteristics in South African Children Hospitalised with Gastroenteritis

- Molecular Detection and Epidemiology of Etiologic Agents among Children with Acute Gastroenteritis at a Secondary Hospital from 2015 to 2018

- Higher abundance of enterovirus A species in the gut of children with islet autoimmunity

- Evaluation of a New Multiplex Real-Time PCR Assay for Detecting Gastroenteritis-Causing Viruses in Stool Samples

- Comparison of three multiplex real-time PCR assays for detection of enteric viruses in patients with diarrhea

- Evaluation of an Assay Based On Multiple Detection Temperature Technique for Simultaneous Detection of Viral Gastroenteritis-Causing Pathogens

Note

[1] Enteroaggregative E. coli

[2] Enteropathogenic E. coli

[3] Enterotoxigenic E. coli

[4] Shiga toxin-producing E. coli

[5] Shigella spp./Enteroinvasive Escherichia coli

[6] For use with Seegene NIMBUS & STARlet only