Gastrointestinal Panel Assays

Gastrointestinal infection can be caused by a wide range of bacterial, viral, and/or parasitic pathogens, and has signs and symptoms such as watery diarrhea, nausea, vomiting, and fever. Real-time PCR method is a more efficient than conventional methods with its high sensitivity and specificity in detecting viral, bacterial, and parasitical pathogens associated with gastrointestinal infections.

Allplex™ Gastrointestinal Panel Assays are a multiplex one-step Real-time RT-PCR assay that detects and identifies 37 GI pathogens, including 6 viruses, 15 bacteria, 6 parasites, and 10 helminths. Based on Seegene’s proprietary MuDT™ technology, this assay makes it possible to report multiple Ct values of each pathogen in a single channel without melting curve analysis.

Up to four of Seegene’s GI assays can be combined in one test, enabling laboratories to tailor panels to their specific needs.

Gastrointestinal Infections

Key Features

The widest coverage of pathogens that can cuase GI infection
- Detection of 37 GI pathogens, including 6 viruses, 15 bacteria, 6 parasites, and 10 helminths.
Full syndromic testing solution available to meet various testing needs with:
- Allplex™ GI-Virus Assay
- Allplex™ GI-Bacteria(I) Assay
- Allplex™ GI-Bacteria(I) plus Assay
- Allplex™ GI-Bacteria(II) Assay
- Allplex™ GI-EB Screening Assay
- Allplex™ GI-Parasite Assay
- Allplex™ GI-Helminth(I) Assay
Fully automated workflow using Seegene STARlet-AIOS
- Full automation from extraction to result analysis with Seegene STARlet-AIOS, ensuring higher efficiency and minimizing manual errors

Specification

Analytes

- Allplex™ GI-EB Screening Assay
  - Campylobacter spp.
  - Clostridium difficile toxin A/B
  - Escherichia coli O157
  - Shigella spp./Enteroinvasive E. coli (EIEC)
  - Shiga toxin-producing E. coli (STEC) (stx1/2)
  - Yersinia enterocolitica
  - Salmonella spp.
  - Internal Control (IC)

- Allplex™ GI-Bacteria(II) Assay
  - Enteroaggregative Escherichia coli (EAEC) (aggR)
  - Enteropathogenic Escherichia coli (EPEC) (eaeA)
  - Escherichia coli O157
  - Enterotoxigenic Escherichia coli (ETEC) (lt/st)
  - Hypervirulent Clostridium difficile
  - Shiga toxin-producing Escherichia coli (stx1/2)
  - Internal Control (IC)

- Allplex™ GI-Parasite Assay
  - Blastocystis hominis
  - Cryptosporidium spp.
  - Cyclospora cayetanensis
  - Dientamoeba fragilis
  - Entamoeba histolytica
  - Giardia lamblia
  - Internal Control (IC)

- Allplex™ GI-Bacteria(I) plus Assay
  - Shigella spp. / Enteroinvasive Escherichia coli (EIEC)
  - Vibrio spp.
  - Aeromonas spp.
  - Clostridium perfringens
  - Campylobacter spp.
  - Yersinia enterocolitica
  - Clostridium difficile toxin B
  - Clostridium difficile toxin A
  - Salmonella spp.
  - Internal Control (IC)

- Allplex™ GI-Bacteria(I) Assay
  - Shigella spp. / Enteroinvasive Escherichia coli (EIEC)
  - Vibrio spp.
  - Aeromonas spp.
  - Campylobacter spp.
  - Yersinia enterocolitica
  - Clostridium difficile toxin B
  - Salmonella spp.
  - Internal Control (IC)

- Allplex™ GI-Helminth(I) Assay
  - Ancylostoma spp.
  - Ascaris spp.
  - Enterobius vermicularis
  - Enterocytozoon spp. / Encephalitozoon spp.
  - Hymenolepis spp.
  - Necator americanus
  - Strongyloides spp.
  - Taenia spp.
  - Trichuris trichiura
  - Internal Control (IC)

- Allplex™ GI-Virus Assay
  - Astrovirus
  - Sapovirus
  - Rotavirus A
  - Norovirus GI
  - Norovirus GII
  - Adenovirus F
  - Exogenous internal control (IC)

Specimens

- Allplex™ GI-EB Screening Assay
  - Human stool

- Allplex™ GI-Bacteria(II) Assay
  - Human stool

- Allplex™ GI-Parasite Assay
  - Human stool

- Allplex™ GI-Bacteria(I) plus Assay
  - Human stool

- Allplex™ GI-Bacteria(I) Assay
  - Human stool

- Allplex™ GI-Helminth(I) Assay
  - Human stool

- Allplex™ GI-Virus Assay
  - Human stool

Compatible Instrumentation (CE-IVD Marked)

Real-time PCR
- CFX96™ Real-time PCR Detection System
- CFX96™ DX System
Automated Extraction
- SEEPREP32™
- NuCiSENS® EasyMag®
- Maelstrom™ 9600
Automated Extraction & PCR Setup
- Seegene STARlet
- Seegene STARlet-AIOS™
- Seegene NIMBUS