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Annealing control primer

Innovative primer technology, ACPTM

ACP™ is an innovative primer system that has specificity of PCR despite of short target specific sequence (10 bp) of primer and produces only accurate results. Advantage of ACP™ can be find. The key features of ACP™ technology arise from the unique structure of the ACP™ that contains a regulator. The regulator is composed of a polydeoxyinosine linker. Since the universal base, inosine, has a much lower Tm value compared with that of the common bases (G, A, T, and C), the regulator forms a 'bubble-like' structure at annealing temperatures and maximizes PCR specificity by preventing the nonspecific binding of the primer to a template.

High sensitivity, specifity and reproducibility

High sensitivity

ACP™ accurately amplifies only the target gene even at very low concentrations of template.

High specificity

ACP™ shows high specificity despite the short size of the target specific sequence (about 10bp). This is possible because of the unique structure of the ACP and the two stage PCR procedure.

High reproducibility

ACP™ gives 99% reproducibility.

Comparison of short / long primer and ACP primer

ACP™ has two priming regions due to its structure and extension proceeds only when the two priming segments (3’-segment and 5’-segment) are perfectly matched with the sequence of a template.

In case of target sequence of 10 mer

Lane 1: Short target specific primers (10~14bp), which require a low annealing temperature to be amplified, do not produce a target product.

Lane 2: For the high annealing temperature required for high specificity of PCR, a universal sequence tail is sometimes attached to short target specific primers. However, this often produces false products due to the presence of the universal sequence.

Lane 3: The unique structure of the ACP™ allows amplification of only the target gene in a fast two PCR step protocol that permits high annealing temperatures.

*Lane1: Short primer(only target 10 mer) / Lane2: Longer primer(target 10 mer+tail) / Lane3: ACP(10 mer+regulator+tail) / M: 100bp size marker

In case of target sequence of 19 mer

In addition to the random PCR with short target specific sequence primers, ACP™ gives better PCR results than conventional PCR primers (19bp target specific sequence) in ordinary PCR.

* Lane1: Primer(only target 19 mer) / Lane2: ACP(19 mer+regulator+tail) / M: 100bp size marker