Annealing control primer
ACP™ is innovative primer system that has specificity of PCR despite of short target specific sequence (10 bp) of primer and produce only accurate results. Advantage of ACP™ can be find. The key features of ACP™ technology arise from the unique structure of the ACP™ that contains a regulator. The regulator is composed of a polydeoxyinosine linker. Since the universal base, inosine, has a much lower Tm value compared with that of the common bases (G, A, T, and C), the regulator forms a 'bubble-like' structure at annealing temperatures and maximizes PCR specificity by preventing the nonspecific binding of the primer to a template.
ACP™ accurately amplifies only the target gene even at very low concentrations of template.
ACP™ shows high specificity despite the short size of the target specific sequence (about 10bp). This is possible because of the unique structure of the ACP and the two stage PCR procedure.
ACP™ gives 99% reproducibility.
ACP™ has two priming regions due to its structure and extension proceeds only when the two priming segments (3’-segment and 5’-segment) are perfectly matched with the sequence of a template.
Lane 1: Short target specific primers (10~14bp), which require a low annealing temperature to be amplified, do not produce a target product.
Lane 2 : For the high annealing temperature required for high specificity of PCR, a universal sequence tail is sometimes attached to short target specific primers. However, this often produces false products due to the presence of the universal sequence.
Lane 3 : The unique structure of the ACP™ allows amplification of only the target gene in a fast two PCR step protocol that permits high annealing temperatures.
＊Lane1: Short primer(only target 10 mer) / Lane2: Longer primer(target 10 mer+tail) / Lane3: ACP(10 mer+regulator+tail) / M: 100bp size marker
In addition to the random PCR with short target specific sequence primers, ACP™ gives better PCR results than conventional PCR primers (19bp target specific sequence) in ordinary PCR.
＊ Lane1: Primer(only target 19 mer) / Lane2: ACP(19 mer+regulator+tail) / M: 100bp size marker