Seeplex® Leukemia BCR/ABL

Detection of BCR/ABL fusion gene from blood and bone marrow

  • GEL
NOT AVAILABLE IN THE US

Outline

The Philadelphia chromosome is formed by fusion of ABL (Ableson) proto-oncogene on chromosome 9 with the BCR (Breakpoint Cluster Region) gene on chromosome 22. The BCR/ABL fusion turns out to be positive in 90~95 % of CML (Chronic Myelogenous Leukemia) patients, 25 % of adult ALL (Acute Lymphocytic Leukemia) patients and 5 % of child ALL (Acute Lymphocytic Leukemia) sufferers. Depending on the breakpoint of the BCR gene, different types of BCR/ABL fusion genes (major type: b2a2, b3a2, minor type: e1a2, micro type: c3a2, etc.) are generated. Chromosome tests usually have a high false negative rate as well as unexplainable mosaic forms of chromosome and require a sufficient amount of cells at division stage and require significant time and effort. Even though RT-PCR can be performed even with a limited amount of specimen, using conventional primers require additional nested PCR steps to detect BCR/ABL after initial RT-PCR. Seeplex® Leukemia BCR/ABL detects 8 types of the BCR/ABL fusion gene simultaneously with only one PCR reaction after cDNA synthesis using DPO™ technology, which maximizes specificity and sensitivity.

Features

  1. BCR/ABL gene rearrangement test Test 8 types (b2a2, b3a2, e1a2, c3a2, b1a1, b3a3, b2a3, e1a3) depending on BCR/ABL gene arrangement using single PCR after the reverse transcription.
  2. A single round PCR process reduces the carryover contamination risk, and 8-MOP, which is included in product, eliminates the contamination risk from PCR products
  3. Multiplex PCR with high sensitivity and specificity by applying DPO™ (Dual Priming Oligonucleotide) technology
  4. Accurate system for result interpretation included Internal Control and Positive Control

Analytes

- b2a2
- b3a2
- e1a2
- c3a2
- b1a1
- b3a3
- b2a3
- e1a3

Specimens

- Whole blood - Bone marrow

Result Preview

ID Result
1 e1a2.
2 -*
3 b3a2
4 c3a2
5 b2a2
6 -*
7 b2a2
M: Marker
1~7: Clinical samples
N: Negative Control
*Not detected

Ordering Information

Product Cat. No. Size
Seeplex® Leukemia BCR/ABL BC1110Z 25 rxns

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GEL : Agarose gel
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