Thrombosis is the formation of a blood clot inside a blood vessel, obstructing the blood flow of the cardiovascular system. Several thrombosis associated single nucleotide polymorphisms (SNPs) have been identified and reported to significantly increase the risk of venous thrombosis. One SNP (G20210A) in the Factor II gene has been found to be associated with increased prothrombin levels and an increase in the risk for venous thrombosis in heterozygotes. Three SNPs (R506Q, H1299R and Y1702C) in the factorⅤ genes are one of the most important genetic risk factors for inherited thrombophilia. Two SNPs (C677T and A1298C) in the MTHFR gene cause reduction of MTHFR enzyme activity and heterozygosity for both C677T and A1298C is considered to be a risk factor for venous thrombosis. Anyplex™ II Thrombosis SNP Panel Assay is designed to detect and discriminate six SNPs (1 in Factor II, 3 in Factor V and 2 in MTHFR) in a single tube using the mTOCE™ technology.