- High specificity without production of any non-specific or false-positive results
- Reliable, rapid, practical and cost-effective detection method
- Specific and simultaneous detection of multiple pathogens without any false results
- Decide genotype and subtype of various pathogens. Multiple-SNP Detection
- Specific and simultaneous analysis of multiple single nucleotide polymorphic (SNP) sites
Occurrence of Single nucleotide polymorphisms (SNPs) are responsible for drug resistance leading to various diseases, but additional steps after amplification of an SNP-containing region such as RFLP, sequence or hybridization are needed to identify SNP genotyping accurately. As a result, the longer time will take for SNP genotyping and additional test will be done. High specificity of DPO enables SNP genotyping with only a single PCR by identifying SNP accurately.
- Hetero type of allele 1 & 2: lanes 1~3
- Homo type of allele 1: lanes 4~6
- Homo type of allele 2: lanes 7~9
SNP in CYP2C19 results from a single base pair substitution (681 bp:G→A) at position 681 in exon 5 of CYP2C19 and causes a non-functional protein and affects the metabolism of a number of commonly used drugs. Multiplex PCR analysis of nine human genomic DNA samples with known genotypes at the CYP2C19 locus using conventional primers (left) and DPO™ (right) was carried out. As a result, DPO™-based multiplex PCR clearly distinguished between the different alleles of CYP2C19, while conventional primer-based multiplex PCR did not.