ACP™ Technology

Annealing control primer

ACP™ Technology (Research use only)

ACP™ is innovative primer system that has specificity of PCR despite of short target specific sequence (10 bp) of primer and produce only accurate results. Advantage of ACP™ can be find. The key features of ACP™ technology arise from the unique structure of the ACP™ that contains a regulator. The regulator is composed of a polydeoxyinosine linker. Since the universal base, inosine, has a much lower Tm value compared with that of the common bases (G, A, T, and C), the regulator forms a 'bubble-like' structure at annealing temperatures and maximizes PCR specificity by preventing the nonspecific binding of the primer to a template.

Reference
In-Taek Hwang et al. Annealing control primer system for improving specificity of PCR amplification.
BioTechniques 2003, 35: 1180-1184.

Features of the technology

Advantage of ACP™

1.High sensitivity
It accurately amplifies only the target gene even at very low concentrations of template

2.High specificity
It shows high specificity despite the short size of the target specific sequence (about 10bp). This is possible because of the unique structure of the ACP and the two stage PCR procedure.

3.High reproducibility
It gives 99% reproducibility.


ACP™ (Annealing Control Primer)

ACP™ (Annealing Control Primer) 기술

A. Target core sequence
A target sequence region that has a complementary sequence to a template DNA

B. Non-target universal sequence
A non-target sequence region that has a universal sequence

C. Regulator
A regulator that bridges these two regions and is composed of a polydeoxyinosine linker


Comparison of Short Primer & Long Primer and ACP™ Pimer

Short Primer, Long Primer와 ACP™ Pimer 간의 비교

Target secific sequence is 10bp

Target secific sequence is 10bp Lane 1: Short target specific primers (10~14bp), which require a low annealing temperature to be amplified, do not produce a target product. Lane 2 : For the high annealing temperature required for high specificity of PCR, a universal sequence tail is sometimes attached to short target specific primers. However, this often produces false products due to the presence of the universal sequence. Lane 3 : The unique structure of the ACP allows amplification of only the target gene in a fast two PCR step protocol that permits high annealing temperatures.

Target secific sequence is 19bp

Target secific sequence is 19bp In addition to the random PCR with short target specific sequence primers, ACP gives better PCR results than conventional PCR primers (19bp target specific sequence) in ordinary PCR.

Application

    -development of biomarker other than environment and disease
    -genetic research at the developmental stage
    -genetic research related to biological signal transduction
    -research gene of transgenic and knock-out model
    -research function of gene
    -research genetic mutation
    -analyze gene expression level
    -Tissue-specific RNA polymorphism

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