Annealing control primer
It accurately amplifies only the target gene even at very low concentrations of template
It shows high specificity despite the short size of the target specific sequence (about 10bp). This is possible because of the unique structure of the ACP and the two stage PCR procedure.
It gives 99% reproducibility.
A. Target core sequence
A target sequence region that has a complementary sequence to a template DNA
B. Non-target universal sequence
A non-target sequence region that has a universal sequence
A regulator that bridges these two regions and is composed of a polydeoxyinosine linker