| Method |
DNA
digestion |
Ligation |
Nonspecific
Products
(false positive) |
Limitation |
Sensitivity |
DNA
Walking |
No |
No |
No |
No |
high |
|
| Tail PCR |
No |
No |
From single arbitrary
tail primer |
co-amplification of
specific & nonspecific
products |
high |
|
Inverse PCR
(IPCR) |
yes |
self-ligation
(circulation) |
From nonligated DNA |
1. restriction site
2. intermolecular ligation |
low |
|
Vectorette- or
Splinkerette PCR |
yes |
vectorette
unit ligation |
From ¢®E¡Ëc¢®Ecend-repair
priming¢®E¡Ëc?
(or single
adaptor primer) |
1. restriction site
2. adaptor ligation |
medium |
|
Capture PCR
(CPCR) |
yes |
linker
ligation |
From single
biotinylated primer |
1. restriction site
2. immobilization |
medium |
|
Adaptor-
ligated PCR |
yes |
adaptor
ligation |
From single adaptor
primer amplification |
1. restriction site
2. adaptor ligation |
medium |
|
