DPO™ Technology, Super Multiplex PCR!
Novel Oligo Platform for PCR: DPO™



Successful PCR starts with proper priming between an oligonucleotide primer and the
template DNA. However the inevitable risk of mismatched priming cannot be avoided
in the currently used primer system, even through considerable time and effort are
devoted to primer design and optimization of reaction conditions.
A novel DPO™ system that is structually and functionally different from the primer
system currently in wide-spread use blocked extension of non-specially primed
templates, and thereby generates consistently high PCR specificity even under
less than optimal PCR conditions.

Reference
Jong-Yoon Chun et al. Dual priming oligonucleotide system for the multiplex
detection of respiratory viruses and SNP genotyping of CYP2C19 gene. Nucleic Acids
Research 2007;35(6):e40. Epub 2007 Feb 7.

Kyoung Ho Roh et al. Comparison of the Seeplex Reverse Transcription PCR Assay
with the R-mix Viral Culture and Immunofluorescence Techniques for Detection of Eight
Respiratory VIruses. Annals of Clinical & Laboraroty Science, Vol. 38, No. 1, p. 41-46,
2008.

Jong-Kee Kim et al. Direct detection of lamivudine-resistant hepatitis B virus mutants
by a multiplex PCR using dual-priming oligonucleotide primers. Journal of Virological
Methods 149, 76-84, 2008.

Patents
1. Process Using Dual Specificity Oligonucleotide and Dual Specificity Oligonucleotide
2. Annealing Control Primer and Its Uses


Structure of DPO™


DPO™ comprises of two separate priming regions (a first priming region and a second
priming region) joined by a polydeoxyinosine linker. The linker forms like a "bubble-like
structure" which itself is not involved in priming, but rather delineates the boundary
between two parts of primer.


Features of DPO™

	Freedom in primer design & PCR optimization
	Unparalleled high specificity
	Guaranteed reproducibility
	No primer competition and dimerization in Multiplex
	Single base discrimination
	Broad applications

Principle of DPO^TM

DPO^TM has two functional prming regions (one is longer than the other) separated by the poly (I)
linker. These two unequally distributed priming regions generate dual priming reactions based
on the following scheme, resulting in only target-specific products.

1. Step 1: Poly(I) linker activation
Deoxyinosine has a relatively low melting temperature compared to the natural bases, due to
weaker hydrogen bonding so that the poly (I) linker will form a bubble-like structure at a certain
annealing temperature and separates a single primer into a two functional regions.


2. Step 2: First priming reaction
The longer 5'-segment preferentially binds to the template DNA and initiates "stable annealing".
It acts as a Stabilizer".



3. Step 3: Second priming reaction
The short 3'-segment selectively binds to a target site and determines "target-specific extension".
It acts as a "Determiner".