ACP¢â (Annealing Control Primer) Technology

ACP¢â technology is a novel primer designed to improve the specificity of PCR amplification.
ACP¢â is innovative primer system that has
specificity of PCR despite of short target specific
sequence (10 bp) of primer and produce only
accurate results.

The key features of ACP¢â technology arise from
the unique structure of the ACP¢â that contains a
regulator. The regulator is composed of a
polydeoxyinosine linker. Since the universal base,
inosine, has a much lower Tm value compared
with that of the common bases (G, A, T, and C),
the regulator forms a 'bubble-like' structure at
annealing temperatures and maximizes PCR
specificity by preventing the nonspecific binding
of the primer to a template.


Reference
In-Taek Hwang et al. Annealing control primer system for imprving specificity of PCR amplification. BioTechniques 2003, 35: 1180-1184.




Figure 1-An example

Lane 1: Short target specific primers (10~14bp), which
require a low annealing temperature to be amplified, do
not produce a target product.

Lane 2 : For the high annealing temperature required
for high specificity of PCR, a universal sequence tail is
sometimes attached to short target specific primers.
However, this often produces false products due to the
presence of the universal sequence.

Lane 3 : The unique structure of the ACP allows
amplification of only the target gene in a fast two PCR
step protocol that permits high annealing temperatures.


Figure 2 In addition to the random PCR with short target
specific sequence primers, ACP gives better PCR results
than conventional PCR primers (19bp target specific
sequence) in ordinary PCR.
High sensitivity
It accurately amplifies only the target gene even at very low concentrations of template
High specificity
It shows high specificity despite the short size of the target specific sequence (about 10bp). This is possible because of the unique structure of the ACP and the two stage PCR procedure.
High reproducibility
It gives 99% reproducibility.






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