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SNP Detection
Detection of the C677T SNP of
the MTHFR gene
Seeplex® MTHFR C677T ACE is
designed to detect C677T SNP of the MTHFR gene from blood samples
5X MT C677T
ACE PM
100 ul Primer Mixture (PM)
- Primer pairs for MTHFR C677T
2X Multiplex
Master Mix
250 ul DNA polymerase
Buffer containing dNTPs (dATP, dCTP, dGTP, dTTP)
MgCl2 and stabilizers
8-MOP Solution 75 ul System to prevent carry-over contamination
MT C677T
ACE Marker
50 ul Amplicon size marker
- Marker is used to identify the approximate size of target product run on agarose gel
MT C677T
ACE PC
15 ul Positive Control (PC)
- Mixture of MTHFR C677T clones
User manual 1  
The reagents contained in one set are sufficient for 25 determinations.
- Short term storage : 4℃
- Long term storage : -20℃


In case of the 2X Multiplex Master Mix, 4℃ for short-term storage and -20℃ for long-term storage are recommended. Avoid repetitive thawing/freezing as it may decrease the activity of the Master Mix.
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