|
|
|
|
|
Somatic Mutation Detection
|
Detection of BCR/ABL fusion gene
from blood and bone marrow |
Seeplex® Leukemia BCR/ABL is designed to
detect the BCR/ABL fusion
gene [t(9;22)(q34;q11)
from blood and
whole bone marrow.
(Analyzing product, DNA amplification )
|
|
|
   |
|
|
|
NOT AVAILABLE IN THE US
|
The Philadelphia chromosome is formed by fusion of ABL (Ableson) proto-oncogene on chromosome 9 with the BCR (Breakpoint Cluster Region) gene on chromosome 22. The BCR/ABL fusion turns out to be positive in 90~95 % of CML (Chronic Myelogenous Leukemia) patients, 25 % of adult ALL (Acute Lymphocytic Leukemia) patients and 5 % of child ALL (Acute Lymphocytic Leukemia) sufferers. Depending on the breakpoint of the BCR gene, different types of BCR/ABL fusion genes (major type: b2a2, b3a2, minor type: e1a2, micro type: c3a2, etc.) are generated.
Chromosome tests usually have a high false negative rate as well as unexplainable mosaic forms of chromosome and require a sufficient amount of cells at division stage and require significant time and effort. Even though RT-PCR can be performed even with a limited amount of specimen, using conventional primers require additional nested PCR steps to detect BCR/ABL after initial RT-PCR.
Seeplex® Leukemia BCR/ABL detects 8 types of the BCR/ABL fusion gene simultaneously with only one PCR reaction after cDNA synthesis using DPO™ technology, which maximizes specificity and sensitivity.
- Detection limit:
10 copies (100% detection),
5 copies (80% detection) |
 |
| A. |
BCR/ABL gene rearrangement test
Test 8 types (b2a2, b3a2, e1a2, c3a2, b1a1, b3a3, b2a3, e1a3) depending on BCR/ABL gene arrangement using single PCR after the reverse transcription.
|
| B. |
Contamination Prevention System |
| |
One-time PCR reduces the carryover contamination risk, and 8-MOP, which is included in product, eliminates the contamination risk from PCR. |
|
| |
| C. |
Contamination Prevention System |
|
One-time PCR reduces the carryover contamination risk, and 8-MOP, which is included in product, eliminates the contamination risk from PCR. |
| D. |
Accurate system for interpretation of results |
|
Internal Control and Positive Control are included in product. |
|
 |
|
 |
- b2a2
-
b3a2
-
e1a2
-
c3a2 |
-
b1a1
-
b3a3
-
b2a3
-
e1a3 |
|
- Whole blood
- Bone marrow |
|
|
 |
| Product |
Cat. No. |
Size |
| Seeplex® Leukemia BCR/ABL |
BC1110Z |
25 rxns |
|
|
|
|
- As this product is Medical Device, please read 'caution' and 'instruction' before use.
- Deliberation number : 2013-I10-01-0847
|
|
|
|
|
|
