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SNP Detection
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| CYP2C19 Genotyping |
Seeplex® CYP2C19 ACE Genotyping is
designed to detect the CYP2C19 *2 and *3
alleles from blood samples.
(Analyzing product, DNA amplification )
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NOT AVAILABLE IN THE US
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Cytochrome P450 2C19 (CYP2C19) is involved in the metabolism of several therapeutic agents, such as anticonvulsants, antidepressants, proton pump inhibitors, HIV-protease inhibitors and antimalarial drugs1). However, if a single point mutation occurs in the CYP2C19 gene, it produces an inactive CYP2C19 enzyme; in turns, the metabolic rates of the drugs are decreased and thus undesirable side effects at standard dosages may arise.
Therefore, identification of the single nucleotide polymorphism (SNP) in the CYP2C19 gene is very important before prescribing medicines. Besides the wild-type CYP2C19 *1 allele, 20 variant CYP2C19 alleles with mutations have been identified and are named as CYP2C19 *2 to *21. Of those mutated alleles, CYP2C19 *2 and CYP2C19 *3 are the most prevalent alleles worldwide. The CYP2C19 *2 and the CYP2C19 *3 alleles are found in ~87 % and ~98% of poor metabolizers (PMs) in Caucasians and Asians, respectively2). By applying an innovative DPO™ based multiplex PCR system having maximized specificity and sensitivity, Seeplex® CYP2C19 ACE Genotyping simply, rapidly, and accurately detects two SNPs (CYP2C19 *2 and CYP2C19 *3 allele) of the CYP2C19 gene without further experiments or expensive instruments.
1) Koori Nakamota et al., Pharmacogenetics and Genomics 2007, 17:103-11 4..
2) Linder MW, ALDES R Jr. Pharmacogenetics in the practice of laboratory medicine. Mol Diagn 1999;4:365-37 9.. |
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| A. |
Detection of Single Nucleotide Polymorphism (SNP) in the CYP2C19 gene
with single PCR reaction |
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Single point substitution causes the wild gene (CYP2C19*1) to change either into
CYP2C19*2 or CYP2C19*3 with one-time PCR reaction. |
| B. |
Accurate system for interpretation of results |
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Internal control is included in each set of product. |
| C. |
Contamination Prevention System |
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One-time PCR reduces the carryover contamination risk, and 8-MOP, which is included in product, eliminates the contamination risk from PCR. |
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| D. |
Applicable for Auto-capillary Electrophoresis device |
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PCR amplicons are separated and analyzed automatically; consequently, rapid and easy interpretation of the results is possible. |
| E. |
Multiplex PCR with high sensitivity and specificity by applying DPO™ (Dual Priming Oligonucleotide) technology |
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DPO™ based primers contain two priming parts so that only target gene is specifically amplified. |
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| - Whold blood |
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- CYP2C19 *1 (Wild type)
- CYP2C19 *2 (681A)
- CYP2C19 *3 (636A) |
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ScreenTape®
System
(Agilent
Technologies)
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MCE®-202
MultiNA
SHIMADZU
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| Product |
Cat. No. |
Size |
| Seeplex® CYP2C19 ACE Genotyping |
CY1210Z |
25 rxns |
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- As this product is Medical Device, please read 'caution' and 'instruction' before use.
- Deliberation number : 2013-I10-01-0839
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