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TOCE™ is a novel technology for melting curve analysis. The method is readily utilizable for organism identification and the detection of mutations in clinical samples. Until now, melting curve analysis has been constrained by several inherent limitations – namely, constrained probe design, the inability to extensively and efficiently multiplex, and high sensitivity of the melting curves (Tm) due to sequence variation of the probe site.
TOCE™ technology overcomes these limitations, providing a new concept of melting curve analysis enhanced multiplexing, improved flexibility in probe design and cross-platform compatibility.
Applicable Items
- Anyplex™ II : STI-7 Detection
- Anyplex™ II : RV16 Detection
Application of
Anyplex™ II system.
1.
Multiplicity in a single fluorescence channel
2.
Consistency in Tm value regardless of the sequence variation
3.
As sensitive as singleplex Real-time PCR
4.
Identical efficiency between single infection and co-infection
1. Multiplicity in a single fluorescence channel
TOCE™ technology can identify multiple pathogens simultaneously in a single fluorescence channel.
Application of
Anyplex™ II system.
1.
Multiplicity in a single fluorescence channel
2.
Consistency in Tm value regardless of the sequence variation
3.
As sensitive as singleplex Real-time PCR
4.
Identical efficiency between single infection and co-infection
2. Consistency in Tm value regardless of the sequence variation
In case of Influenza A, Human rhinovirus and Human adenovirus that have high sequence variation, sequence differences can only be observed at maximum of 13ºC by melting curve analysis. In contrast, TOCE™ technology is not affected by sequence variations, thereby guarantees consistent Tm values.
Application of
Anyplex™ II system.
1.
Multiplicity in a single fluorescence channel
2.
Consistency in Tm value regardless of the sequence variation
3.
As sensitive as singleplex Real-time PCR
4.
Identical efficiency between single infection and co-infection
3. As sensitive as singleplex Real-time PCR
TOCE™ technology provides the same level of sensitivity as probe-based singleplex Real-time PCR.
Application of
Anyplex™ II system.
1.
Multiplicity in a single fluorescence channel
2.
Consistency in Tm value regardless of the sequence variation
3.
As sensitive as singleplex Real-time PCR
4.
Identical efficiency between single infection and co-infection
4. Identical efficiency between single infection and co-infection
Amplifying multiple pathogens using TOCE™ technology is as efficient as single pathogen amplification.
