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ACP™ technology is a novel primer designed to improve the specificity of PCR amplification.

ACP™ is innovative primer system that has specificity of PCR despite of short target specific sequence (10 bp) of primer and produce only accurate results. The key features of ACP™ technology arise from the unique structure of the ACP™ that contains a regulator. The regulator is composed of a polydeoxyinosine linker. Since the universal base, inosine, has a much lower Tm value compared with that of the common bases (G, A, T, and C), the regulator forms a 'bubble-like' structure at annealing temperatures and maximizes PCR specificity by preventing the nonspecific binding of the primer to a template.

Reference
In-Taek Hwang et al. Annealing control primer system for improving specificity of PCR amplification. BioTechniques 2003, 35: 1180-1184.
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Super Multiplex PCR
1. High sensitivity
It accurately amplifies only the target gene even at very low concentrations of template
2. High specificity
It shows high specificity despite the short size of the target specific sequence (about 10bp). This is possible because of the unique structure of the ACP and the two stage PCR procedure.
3. High reproducibility
It gives 99% reproducibility.
 
ACP™ (Annealing Control Primer)

A.Target core sequence
A target sequence region that has a complementary sequence to a template DNA

B. Non-target universal sequence A non-target sequence region that
has a universal sequence

C. Regulator A regulator that bridges these two regions
and is composed of a polydeoxyinosine linker
 
Comparison of various primer
Target secific sequence is 10bp

Lane 1: Short target specific primers (10~14bp), which require a low annealing temperature to be amplified, do not produce a target product. Lane 2 : For the high annealing temperature required for high specificity of PCR, a universal sequence tail is sometimes attached to short target specific primers. However, this often produces false products due to the presence of the universal sequence. Lane 3 : The unique structure of the ACP allows amplification of only the target gene in a fast two PCR step protocol that permits high annealing temperatures.
Target secific sequence is 19bp

Figure 2 In addition to the random PCR with short target specific sequence primers, ACP gives better PCR results than conventional PCR primers (19bp target specific sequence) in ordinary PCR.
Application
  • A. Applicable to all eukaryotic organisms


  • B. Applicable to all differentially expressed genes
         - Research into disease related genes
         - Research into genes that respond to specific drug/hormone treatments
         - Research into genes whose transcription changes with the biological            process/environment
         - Research into genes involved in development
         - Research into genes involved in cell differentiation
         - Research into genes involved in signal transduction
         - Research into biomarkers


  • C. Integration site identification
         - Determination of location or orientation of transgene/virus/transposon
         - Screening of deletion or insertion


  • D. Unknown sequence identification
         - Full-length cDNA cloning or sequencing of EST
         - Cloning of promoter regions
         - Large clone sequencin
         - BAC/YAC clone sequencing
         - Splicing analysis

    E. Functional studies of specific genes
    F. Screening of alternatively spliced variants of specific genes
    G. Study of genetic mutations in specific genes
    H. Analysis of the expression levels of tissue specific genes
    I. Tissue-specific RNA polymorphism
    J. Follow-up studies after the GeneFishing service
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