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| DNA Walking kit I |
K1501 |
K1502 |
| 1 |
2.5μM DW-ACP 1 primer for the first PCR reaction |
DW-ACP 1: 5’-ACP-AGGTC-3 |
40μl |
50μlX2 |
| 2 |
2.5μM DW_ACP 2 primer for the first PCR reaction |
DW-ACP 2: 5’-ACP-TGGTC-3 ’ |
40μl |
50μlX2 |
| 3 |
2.5μM DW_ACP 3 primer for the first PCR reaction |
DW-ACP 3: 5’-ACP-GGGTC-3’ |
40μl |
50μlX2 |
| 4 |
2.5μM DW-ACP 4 primer for the first PCR reaction |
DW-ACP 4: 5’-ACP-CGGTC-3’ |
40μl |
50μlX2 |
| 5 |
10μM DW-ACP N primer for the second PCR reaction |
DW-ACPN: 5’-ACPN-GGTC-3’ |
40μl |
50μlX2 |
| 6 |
10μM universal primer for the third PCR reaction |
Uni-primer: 5’-
TCACAGAAGTATGCCAAGCGA-3 |
40μl |
50μlX2 |
2X
Master Mix |
2X SEEAMP™ ACP™ Master Mix II (1ml) |
|
2ea |
5ea |
| DNA Walking kit II |
| 1 |
5μM DW2-ACP1 primer for the first PCR reaction |
DW2-ACP 1: 5’-ACP-AACGG-3 |
30μl |
| 2 |
5μM DW2-ACP2 primer for the first PCR reaction |
DW2-ACP 2: 5’-ACP-CTCGA-3 |
30μl |
| 3 |
5μM DW2-ACP3 primer for the first PCR reaction |
DW2-ACP 3: 5’-ACP-CTACG-3 |
30μl |
| 4 |
5μM DW2-ACP4 primer for the first PCR reaction |
DW2-ACP 4: 5’-ACP-ACGTG-3 |
30μl |
| 5 |
5μM DW2-ACPN primer for the second PCR reaction |
DW2-ACPN: 5’-ACPN-3 |
120μl |
| 6 |
5μM universal primer for the third PCR reaction |
Uni-primer2: 5’-
GAGTTTAGGTCCAGCGTCCGT-3 |
60μl |
2X
Master Mix |
2X SEEAMP™ ACP™ Master Mix II (1ml) |
|
2ea |
