GeneXp¢â kit is Seegene¡¯s original DPO¢â -based multiplex RT-PCR system.
- Only a single reaction in necessary to amplify multiple genes of interest.
- One reaction is sufficient to amplify multiple genes simultaneously in a single reaction.
GeneXP¢â kit uses target specific primers for target gene profiling.
Multiplex PCRs capacity to simultaneously quantify miltiple mRNA species from a single of RNA allows comparative analysis of different mRNA species within samples. Variations in RNA isolation, initial quantification errors or tube-to-tube variations in RT and PCR can be compensated by including a known housekeeping gene.
DPO¢â primers can simultaneously detect multiple target genes without creating any false products.
Compare with the other method To date, gene expression patterns are often analyzed by microarray methods that involve a DNA chip or by PCR-based methods.
Microarray Microarray analysis has the advantage over the other methods in that it permits high throughput screening at one time. However, it has many potential pitfalls, such as a high false-positive rate and low sensitivity. Moreover, the number of samples that can be compared is restricted, and it is difficult to detect subtle differences between the samples.
Experiments need to be repeated due to the low reproducibility Hybridization method is comparably lower in sensitivity than PCR method Additional experiments (Northern blot, Real-time PCR, RT-PCR, etc.)are required for validation. All the clones with positive signal need to be checked, hence requiring more cost and time for confirmation steps
GeneXp¢â Kit Systematic Analysis of Gene Family Expression High Specificity & Sensitivity using by DPO¢â-based multiplex RT-PCR No more confirmation step required
Comparison of normal vs. diseased tissue Ex. normal vs. tumor, non-infected vs. infected, wild type vs. knock-out
Time courses Ex. developmental stage, environmental change
Test of influence of Chemical compounds Ex. drug, chemical, hormone, post-clinical treatments
Microarray data validation, etc.
Cat.
No
Product
Target Gene
HK16XX
GeneXp¢â Human Prognosis Signature Assay Kit (1~11)
Microarray analysis has the advantage over the other methods in that it permits high throughput screening at one time. However, it has many potential pitfalls, such as a high false-positive rate and low sensitivity. Moreover, the number of samples that can be compared is restricted, and it is difficult to detect subtle differences between the samples. 
