However, conventional method of detecting sepsis-causing pathogens - blood culture - is so
time-consuming that it is not appropriate for a patient in intensive care unit or an emergency
room. Among sepsis-causing pathogens, fungi are especially difficult to detect by blood
culture and thus prescribing proper medicine is delayed, causing the death of patient.
Moreover, excess administration of initial broad-spectrum antibiotics treatment may result in
developing resistance to antibiotics.

Unlike the blood culture method, Seeplex^® Sepsis DNA Test makes it possible to do early screening for sepsis.

The conventional method for sepsis diagnostics is blood culture; However, it takes up to 8
days for the result; therefore it is not apropriate for a patient in an intensive care unit or an
emergency room.
The Proprietary technology of Seeplex^® Sepsis DNA Test does not require for the blood
culture process and enables precise one-step screening for complex range of pathogens
at the earliest stage of disease development. This product can screen for 64 sepsiscausing
pathogens in total - 48 Gram (+) bacteria, 10 Gram (-) bacteria, and 6 fungi simultaneously,
leading to saving great amount of time. Additionally, 3 drug resistance genes (vanA, vanB,
and mecA) can also be discriminated.


Key Features:
- One-step screening for 64 sepsis-causing pathogens from whole blood, periodontal and caries joint fluid, cerebrospinal fluid, blood cultures, urine, sputum, and other body fluids
- Discrimination of 3 drug resistance genes
- Seeplex^® PCR with high specificity and sensitivitya
- Directly from 0.4 ml of whole blood
- 5 hours of turnaround time
- Low cost
- Application on high throughput auto-detection systems


Target Pathogens (64)
Fungi (6)
Staphylococcus spp. (22)
Enterococcus spp. (2)
Gram negative bacteria (10)
Streptococcus spp. (24)

Drug Resistance Genes (3)
vanA, vanB, and mecA


Table 1. Group Screening list



Fig. 1 & Table 2. Examples


Click the image for see large image

Fig. 1 & Table 2. Amplified PCR products using the Seeplex^® Sepsis Screening Set
were electrophoresed on auto electrophoresis system (Rapid & easy interpretation
using ScreenTape^® System).
* Gram negative bacteria 1 band includes Klebsiella pneumoniae, Klebsiella oxytoca
Serratia marcescens, Enterobacter cloacae and Enterobacter aerogenes.
* Gram negative bacteria 2 band includes Escherichia coli, Proteus mirabilis
Pseudomonas aeruginosa, Acinetobacter baumannii, and
Stenotrophomonas maltophilia.


Sepsis ID Set

The Seeplex^® Sepsis ID Set can identify 25 sepsis-causing pathogens from whole blood,
periodontal and caries joint fluid, cerebrospinal fluid, blood cultures, urine, sputum, and
other body fluids.

Table 3. Identification list


Fig. 2 & Table 4. Examples


Click the image for see large image

Fig. 2 & Table 4. Amplified PCR products using the Seeplex^® Sepsis ID Set were
electrophoresed on auto electrophoresis system (Rapid & easy interpretation using
ScreenTape^® System).


Sepsis Drug Resistance

The Seeplex^® Sepsis Drug Resistance can discriminate 3 drug resistance genes such as
vanA, vanB, and mecA by using multiplex PCR.

Fig. 3 & Table 5. Example

.
Fig. 3 & Table 5. Amplified PCR products using the Seeplex^® Sepsis Drug Resistance
were electrophoresed on auto electrophoresis system (Rapid & easy interpretation
using ScreenTape^® System).