Simultaneously detecting 12 major respiratory viruses.
The Seeplex® RV12 ACE Detection for auto-capillary electrophoresis is designed to detect 12 major respiratory viruses, 11 respiratory RNA viruses and 1 DNA virus, from patients' samples including nasopharyngeal aspirates, nasopharyngeal swabs and bronchoalveolar lavage. The conventional methods (culture and IFA, etc.) to detect respiratory viruses takes several days or do not have enough sensitivity. Therefore, many of the recently developed diagnostic methods employ PCR methods. Among them, Seegene's Respiratory Virus Detection is outstanding in terms of sensitivity and specificity by applying DPOTM technology and detects the largest numbers of respiratory viruses.
Introducing a useful reference for diagnosis of respiratory viruses infection: Grunteke P. et al. Practical Implementation of a multiplex PCR for acute respiratory tract infections in children. J Clin Microbiol. 2004. 42(12):5596-5603. Target pathogens: Influenza A virus (Flu A) Influenza B virus (Flu B) Respiratory syncytial virus A (RSV A) Respiratory syncytial virus B (RSV B) Parainfluenza virus 1 (PIV 1) Parainfluenza virus 2 (PIV 2) Parainfluenza virus 3 (PIV 3) Coronavirus 229E/NL63 (OC229E/NL63) Coronavirus OC43/HKU1 (OC43/HKU) Rhinovirus A/B (HRV A/B) Adenovirus (AdV) Metapneumovirus (MPV) Figure & Table. Example (ScreenTape® System)    Click the image for large view. Figure & Table. Amplified PCR products using the Seeplex® RV12 ACE Detection. (Rapid & easy interpretation using ScreenTape® System) Features - Simultaneous detection of 12 prevalent respiratory viruses ( 11 respiratory RNA viruses & 1 DNA virus) - Multiplex RT-PCR with high sensitivity and specificity by applying DPO™ (Dual Priming Oligonucleotide) technology DPO™ (NET* approved) based primers contain two priming parts so that only target gene is specifically amplified. *NET (New Excellent Technology) is approved Korea Ministry of Education, Science, and Technology. - Applicable for Auto-capillary Electrophoresis device PCR amplicons are separated and analyzed automatically; consequently, rapid and easy interpretation of the results is made possible. - Contamination Prevention System One-time PCR reduces the carryover contamination risk, and 8-MOP, which is included in each set of products, eliminates the contamination risk from PCR. - Accurate system for interpretation of results Internal control and positive control are included in each set of products. Advantage of Seeplex® system Detection method Feature Virus Culture Time-consuming, Impossible to genotyping Impossible to early detect Need second identification method Antigen Detection Low sensitivity, Impossible to genotyping Impossible to early detect Hybridization Low sensitivity, Low reliability Oligo Chip Low reproducibility, False signal PCR detection methods High sensitivity and specificity Adequate for early detection and monitering for virus False signal, Limited number of virus detection in one-tube Conventional Multiplex PCR False signal, Low sensitivity Method Multiple analysis Early diagnosis Specificity Sensitivity Testing time ELISA / IFA Possible Impossible Low Low Short Virus Culture Impossible Impossible High Low Long PCR Possible Possible Middle High Short Chip Impossible Possible Low Middle Short Seeplex® system Possible Possible High High Short Order information   Product Cat. No. Size   Seeplex® RV12 ACE Detection RV6C00Y 50 rxns * Note: If you need more information for the primer mixture, please contact us.

* Storage:
Short term storage : 4℃
Long term storage : -20℃
In case of the 2X Multiplex Master Mix, 4 ℃ for short-term storage and -20℃ for long-term
storage are recommended. Avoid repetitive thawing/freezing as it may decrease the activity
of the Master Mix.


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